Nakano LAb. for immunoregulation & HEALTHY AGING

 

PI: Toshiaki Nakano

Professor

Graduate Institute of Clinical Medical Sciences,

Chang Gung University College of Medicine, Taoyuan, Taiwan

Liver Transplantation Center,

Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan

  • About ME

    Interested in Transplant Immunology, Immune Tolerance, Liver Biology, Tumor Immunity, Allergy and Biomarker Discovery for Promotion of Healthy Aging

    Membership

    Japanese Society of Immunology (2004~)

     

    The Transplantation Society (TTS) (2008~)

     

    International Liver Transplantation Society (ILTS) (2012~)

     

    International Liver Cancer Association (ILCA) (2017~)

    International Activity

    Editorial Board Member

    Scientific Reports (2015~)

     

    Guest Editor

    International Journal of Molecular Sciences (2022-2023)

    Special Issue: The Road to Tolerance: Molecular Biomarkers for Prediction and Diagnosis of Post-transplant Complications in Liver Transplantation

    https://www.mdpi.com/journal/ijms/special_issues/S0C576P149

    Honor

    Poster Award

    XX International Congress of TTS (Vienna, 2004)

    ILTS 15th Annual International Congress (New York, 2009)

    ILTS16th Annual International Congress (Hong Kong, 2010)

    ILTS18th Annual International Congress (San Francisco, 2012)

    World Transplant Congress (San Francisco, 2014)

    14th Congress of the Asian Society of Transplantation (Singapore, 2015)

     

    Mentee-Mentor Award

    TTS-ILTS Basic Science Mentor Award (Paris, 2013)

  • appointment

    Professor

    Aug. 2022 to present

    Graduate Institute of Clinical Medical Sciences,

    Chang Gung University College of Medicine

    Associate Professor

    Aug. 2013 to Jul. 2022

    Graduate Institute of Clinical Medical Sciences,

    Chang Gung University College of Medicine

    Associate Research Fellow (Joint Appointment)

    Jan. 2016 to present

    Graduate Institute of Clinical Medical Sciences,

    Chang Gung University College of Medicine

    Assistant Professor

    Aug. 2009 to Jul. 2013

    Department of Surgery,

    Kaohsiung Chang Gung Memorial Hospital

    Assistant Research Fellow

    Jan. 2008 to Jul. 2009

    Department of Medical Research,

    Kaohsiung Chang Gung Memorial Hospital

    Post-doctoral Fellow

    Apr. 2004 to Dec. 2007

    Department of Surgery,

    Kaohsiung Chang Gung Memorial Hospital

  • Education

    Cluster III (Chemistry, Biotechnology and Process Engineering), Fuculty of Enginnering, Hiroshima University, Japan.

    1995 - 1999

    Bachelor of Engineering

    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.

    1999-2001

    Master of Science

    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.

    2001-2004

    Ph.D. of Science

  • Research topics

    Extracellular Nuclear Antigens as Alermins

    Nuclear histone H1, which previously was thought to function only as a nuclear factor that is involved in the formation of higher-order chromatin structures and plays a vital role in transcriptional regulation, was recently highlighted as an important player for regulation of innate and adaptive immunity. First discover of histone H1 as an immune regulator in transplant immunology was published in 2004 (Nakano T, et al. Transplantation 2004). In addition, another nuclear antigen, high-mobility group box 1 (HMGB1), was also discovered as a nuclear weapon in the immune arsenal (Lotze MT, et al. Nat Rev Immunol 2005). Specific antibodies against nuclear histone H1 and HMGB1 are highly expressed not only in experimental models of liver allograft tolerance but also in the patient who accept the donor liver without immunosuppressive therapy (termed as operational tolerance) (Nakano T, et al. Transplantation 2007). Our further study strongly suggest that sensitivity to nuclear antigens such as histone H1 may be one of the key factors determining the acceptance or rejection of donor liver grafts (Nakano T, et al. Transplant Immunol 2008).

    Histone H1 blockage by anti-histone H1 antibody downregulated the intracellular activation of mitogen-activated protein kinases (MAPKs), IkB and NF-kB of dendritic cells (DCs), and inhibited DC activity in the proliferation of CD4+ T cells. In contrast, the addition of histone H1 upregulated major histocompatibility complex class II (MHC II), the CD80 and CD86 surface markers of DCs. These results suggest that the translocation of histone H1 from nuclei to cytoplasm and the release of their own histone H1 are necessary for the maturation of DCs and the activation for T-lymphocytes (Hsu LW, Goto S, Nakano T, et al. Clin Exp Immunol 2008). We speculate that the induction of autoantibody against nuclear antigens may be an “ultimate weapon” to prevent a breakdown of the immune system. In deed, nuclear histone H1 and HMGB1 were detectable in the serum after liver transplantation (Nakano T*, et al. Clin Dev Immunol 2013) and the active induction of de novo autoimmune hepatitis after liver transplantation could prolong liver allograft survival (Nakano T*, et al. Clin Dev Immunol 2013).

    In addition to the impact of histone H1 and corresponding autoantibody in transplant rejection and tolerance, we have recently demonstrated the correlation between circulating histone H1 and OVA-specific IgE levels in a mouse model of OVA-induced allergic rhinitis (Nakano T*, et al. PLoS One 2016). The elevation of histone H1 may cause non-specific allergic response and it must be one of molecular targets for diagnosis and therapeutics.

  • Extracellular GAPDH for Immunomodulation

    GAPDH was accidentally identified as one of the target antigens for anti-histone H1 antibody on T cell surface (see above project). GAPDH, which is constitutively expressed in the cytosol, is a glycolytic pathway-related enzyme and is best known as a housekeeping molecule. GAPDH reversibly catalyzes the phosphorylation of glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate, which is essential for energy metabolism. To explore the impact of GAPDH on inflammatory response, we have established LPS-induced sepsis-related severe acute lung injury model. Briefly, 10 mg/kg of GAPDH was pre-injected before LPS administration and evaluated the survival rate. Surprisingly, we have demonstrated the prevention of LPS-induced septic death by GAPDH pre-treatment (Takaoka Y, Goto S, Nakano T*, et al. Sci Rep 2014).

    To further explore mechanical insights of GAPDH on prevention of LPS-induced septic death, we have explored the impact of GAPDH on macrophages, one of important players at the onset of sepsis and acute lung injury. Macrophages have two opposite phenotypes, which include pro-inflammatory (M1) and anti-inflammatory (M2) statuses. We speculated that GAPDH treatment modulate M1/M2 macrophage polarization. In deed, GAPDH treatment significantly reduced TNF-α production, while it enhanced IL-10 production in LPS-stimulated macrophages. Heat-inactivated GAPDH failed to suppress TNF-α production, suggesting the involvement of enzyme activity. In the course of glycolysis, GAPDH activity produces reduced nicotinamide adenine dinucleotide (NADH). GAPDH treatment enhanced the intracellular level of NADH in LPS-stimulated macrophages similar to IL-4-induced M2 macrophages. Correspondingly, NAD+/NADH ratio was significantly reduced by GAPDH treatment as compared with LPS/IFN-γ-induced M1 macrophages. These results suggest the involvement of NADH formation on M2 macrophage polarization. LPS stimulation may induce proinflammatory cytokines, while it may induce the secretion of GAPDH in macrophages. Extracellular GAPDH modulates intracellular NAD+/NADH balance, resulting in the promotion of anti-inflammatory M2 macrophages for termination of inflammatory response (Nakano T*, et al. Biofactors 2018).

  • microRNAs for Prediction/Diagnosis of Post-transplant Complications

    Liver transplantation is widely accepted as an effective therapeutic modality for end-stage liver diseases. However, post-transplant complications are common in the early and long-term period and contribute to significant morbidity and mortality. Therefore, how we precisely predict/diagnose post-transplant complications such as liver graft dysfunction, acute rejection (AR), infection and hepatitis or hepatocellular carcinoma (HCC) recurrence is a quite important issue for the transplantation society.

    In our previous study, we compared microRNA expression profiles between naïve and AR livers at day 7 after OLT with short- (<14 days, donor DA liver into LEW recipient) and long-term (>60 days, donor DA liver into PVG recipient) survival fates (Nakano T*, et al. OMICS 2017). The microarray analysis revealed that the levels of miR-301a in the lethal AR livers were significantly higher than in naïve and tolerogenic AR livers. The reduced expression of miR-301a in inflamed livers such as Concanavalin A (Con A)-induced autoimmune hepatitis and non-alcoholic steatohepatitis (NASH) suggested a difference between AR and inflammation in terms of miR-301a-mediated molecular events.

    Recently, tumor-derived exosomes are highlighted for diagnostic and therapeutic targets. We speculate that some HCC patients possess unique components in circulating exosomes responsible for HCC development and recurrence. However, the mode of action of circulating exosomes on HCC development and recurrence is not fully understood. In our recent study, we have confirmed the impact of circulating exosomes on HCC development in a rat model of HCC (Nakano T*, et al. Am J Transplant 2019). Microarray revealed unique microRNA profile in HCC serum exosomes and miR-92b was selected as one of highly expressing microRNAs. In addition, in situ hybridization revealed highly intense staining for miR-92b in HCC tissues. Clinically, circulating exosomal miR-92b may be potential biomarkers for early prediction of post-transplant HCC recurrence.

  • Phototherapy and Vitamin D3 in Non-alcoholic Fatty Liver Disease

    Obesity and hepatic steatosis are becoming increasingly common medical problems in the developed world population, and it has been reported that some of potential donor livers present some degree of steatosis. Hepatic steatosis includes alcoholic and non-alcoholic fatty liver disease (NAFLD). Additionally, NAFLD includes both simple fatty liver and non-alcoholic steatohepatitis (NASH), defined by the presence of lobular necroinflammatory activity with or without the presence of perisinusoidal fibrosis on liver biopsy. To date, the only reliable method of differentiating simple steatosis from NASH is by liver biopsy, which is costly and carries some risk to patients.

    Vitamin D is recognized as the “sunshine vitamin.” Most humans depend on sun exposure to satisfy their requirements for vitamin D, but it can be obtained from fortified food, oily fish and vitamin D supplements. Solar ultraviolet B photons are absorbed by 7-dehydro-cholesterol in the skin, leading to its transformation to pre-vitamin D3, which is rapidly converted to vitamin D3. Once formed, vitamin D3 is metabolized in the liver to 25-hydroxyvitamin D3 (25(OH)D3; calcidiol) and then in the kidney to its biologically active form, 1a, 25-dihydroxyvitamin D3 (1,25(OH)2D3; calcitriol). Targher et al. recently reported that NAFLD patients have a marked decrease in serum 25(OH)D3 concentration, which is closely associated with histopathological features of NAFLD (Targher G, et al. Nutr Metab Cardiovasc Dis 2007). However, much remains to be learned about the effects of phototherapy and vitamin D status on the progress of NAFLD. To explore the therapeutic effects of phototherapy on the progress of NASH, artificial sunlight (color temperature 5500 K, color rendition indexes >90; Chang Gung Biotechnology, Taipei, Taiwan) was used for 12 hr/day with animals including choline-deficient and iron-supplemented L-amino acid-defined (CDAA) diet-induced and an obesity-related NASH models.

    In our present study, we have demonstrated that serum apolipoprotein E and low molecular weight-adiponectin levels were gradually reduced and reached the lowest level at fatty liver/NASH stage both in CDAA diet-induced NASH model and in genetically obese model. Phototherapy ameliorated hepatocyte apoptosis, inflammation, fibrosis and insulin/leptin resistance caused by CDAA diet with alteration of the levels of lipid transfer/metabolic proteins and elevation of the circulating active form of vitamin D3. Vitamin D3 supplementation ameliorated NASH progression in CDAA diet-induced NASH model. However, phototherapy failed to ameliorate the obesity and steatosis, suggesting that phototherapy may possess anti-inflammatory/fibrotic activity rather than anti-obesity/steatotic activity.

    These results suggest that serum lipid transfer/metabolic proteins and vitamin D3 status may be effective biomarkers for non-invasive diagnosis of NASH progression, and that phototherapy may be a good complementary therapy for NASH because of its regulation of lipid transfer/metabolic proteins and vitamin D3. This study (Nakano T, et al. J Hepatol 2011) is the first confirmation of therapeutic potency of sunlight therapy and Vitamin D3 supplementation in the animal model of fatty liver disease, which clearly builds the basis for subsequent human therapeutic trials in NAFLD. This finding is highlighted as an Editorial (Geier A. J Hepatol 2011).

  • Phototherapy and Fecal Microbiota Transplantation in Food Allergies

    In our previous study, we have demonstrated the reduced expression of vitamin D3in non-alcoholic steatohepatitis (NASH), and phototherapy with the artificial sunlight (full spectrum, color temperature 5500 K, color rendition index (CRI)>90Ra; Chang Gung Biotechnology, Taipei, Taiwan) and vitamin D3 supplementation ameliorated NASH progression in rats (Nakano T, et al. J Hepatol 2011). In our recent study, we applied phototherapy in food allergies (FAs) because of the increasing prevalence of FAs in westernized countries (Chen PJ, et al. NPJ Biofilms Microbiomes 2021). There is currently no cure for FAs, and the traditional treatment is the avoidance of allergenic foods. Otherwise patients must carry a self-injectable form of epinephrine for emergency treatment of anaphylaxis. Here, we proposed applying phototherapy as an alternative approach for the prevention and treatment of FA-like allergic diarrhea.

    Although the mode of action of phototherapy was not fully clarified in this study, one of the potential mechanisms underlining the beneficial impact of phototherapy could be the improvement in FA-associated vitamin D3insufficiency. Another potential mechanism of phototherapy in the prevention of FA could be the modulation of gut microbiota. We performed fecal microbiota transplantation (FMT) to demonstrate functional roles of microbiota composition in FA. Briefly, FA-associated dysbiosis could directly transfer FA symptoms in naïve BALB/c mice. On the other hand, the replacement of FA-associated bacteria with healthy microbiota may be a promising strategy for FA therapeutics. We identified the genus Lachnospiraceae_NK4A136_group (phylum Firmicutes) as an FA-associated microbacteria. In addition, one of the beneficial bacterial species, Parabacteroides goldsteinii (phylum Bacteroidetes), was enriched in phototherapy-treated mice. Although further studies are necessary, these unique microbes may play some roles in FA development and prevention.

  • Ongoing projects

    The Ministry of Science and Technology (MOST)

    Aug. 2020-Mar. 2022

    Development of innovative diagnostic procedures for post-liver transplant patients: at-home health screening by detection of nuclear antigen release and following molecular diagnosis for precision medicine (MOST109-2320-B-182-014)

    Chang Gung Medical Foundation

    Sep. 2022-

    Vitamin D3 and alarming as molecular targets for prevention and amelioration of food allergy

    (CMRPD8L1121)

    Call For Papers

    -Mar. 31, 2023

    International Journal of Molecular Sciences

    Special Issue: The Road to Tolerance: Molecular Biomarkers for Prediction and Diagnosis of Post-transplant Complications in Liver Transplantation

    https://www.mdpi.com/journal/ijms/special_issues/S0C576P149

  • lab members and collaborators

    Ms. Hui-Peng Tseng (Senior Research Assistant, CGU)

    Dr. Hui-Ying Liu (Dept. of Urology, Kaohsiung CGMH, Ph.D. students in CGU)

     

    Prof. Chao-Long Chen (Superintendent Emeritus, Liver Transplantation Center, Kaohsiung CGMH)

    Prof. Yu-Fan Cheng (Dept. of Diagnostic Radiology, Liver Transplantation Center, Kaohsiung CGMH)

    Dr. Chien-Chih Chen (Dept. of Psychiatry, Kaohsiung CGMH)

    Dr. King-Wah Chiu (Division of Hepato-Gastroenterology, Kaohsiung CGMH)

    Group Photo

    Basic Research Group

    Liver Transplantation Center

    Kaohsiung Chang Gung Memorial Hospital

  • international collaborators

    Histone H1 and Immune Regulation, Antioxidant and Allergic Regulation, Phototherapy

    Hiroshima University, Japan

    Prof. Seiji Kawamoto

    Josai International University, Japan

    Prof. Shigeru Goto

    Prof. Takeshi Goto

    Prof. Naoya Ohmori

    Oita University, Japan

    Prof. Seigo Kitano (President)

    Prof. Masafumi Inomata

  • Publications

    • Original Article * corresponding author
    1. Chen CC, Nakano T, Hsu LW, Chu CY, Huang KT. Early Lipid Metabolic Effects of the Anti-Psychotic Drug Olanzapine on Weight Gain and the Associated Gene Expression. Neuropsychiatr Dis Treat 2022;18:645-657.
    2. Chen PJ, Nakano T*, Lai CY, Chang KC, Chen CL, Goto S. Daily full spectrum light exposure prevents food allergy-like allergic diarrhea by modulating vitamin D3 and microbiota composition. NPJ Biofilms Microbiomes 2021;7(1):41.
    3. Hsu LW, Huang KT, Nakano T, Chiu KW, Chen KD, Goto S, Chen CL. MicroRNA-301a inhibition enhances the immunomodulatory functions of adipose-derived mesenchymal stem cells by induction of macrophage M2 polarization. Int J Immunopathol Pharmacol 2020;34:2058738420966092.
    4. Lin SH, Ho JC, Li SC, Cheng YW, Yang YC, Chen JF, Hsu CY, Nakano T, Wang FS, Yang MY, Lee CH*, Hsiao CC*. Upregulation of miR-941 in Circulating CD14+Monocytes Enhances Osteoclast Activation via WNT16 Inhibition in Patients With Psoriatic Arthritis. Int J Mol Sci 2020;21(12):E4301.
    5. Nakano T*, Chen IH, Wang CC, Chen PJ, Tseng HP, Huang KT, Hu TH, Li LC, Goto S, Cheng YF, Lin CC, Chen CL*. Circulating exosomal miR-92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence. Am J Transplant 2019;19(12):3250-62.
    6. Chiu KW, Goto S, Nakano T, Hu TH, Chen DW, Huang KT, Hsu LW, Chen CL.Genetic polymorphisms of the hepatic pathways of fatty liver disease after living donor liver transplantation. Liver Int 2018;38(12):2287-93.
    7. Nakano T*, Goto S, Takaoka Y, Tseng HP, Fujimura T, Kawamoto S, Ono K, Chen CL. A novel moonlight function of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for immunomodulation. Biofactors 2018;44(6):597-608.
    8. Chiu KW, Nakano T, Chen KD, Hu TH, Lin CC, Hsu LW, Chen CL, Goto S. Identification of IL-28B genotype modification in hepatocytes after living donor liver transplantation by laser capture microdissection and pyrosequencing analysis. BioMed Res Int 2018;2018:1826140.
    9. Chiu KW, Nakano T, Hu TH, Chen KD, Hsu LW, Eng HL, Cheng YF, Goto S, Chen CL. Association between Subclinical Low Serum 25(OH)D in Donors and Fatty Liver Disease in Recipients after Living Donor Liver Transplantation. BioMed Res Int 2018;2018:4508085.
    10. Chen CC, Hsu LW, Huang KT, Goto S, Chen CL, Nakano T*. Overexpression of Insig-2 inhibits atypical antipsychotic-induced adipogenic differentiation and lipid biosynthesis in adipose-derived stem cells. Scientific Reports 2017;7(1):10901.
    11. Nakano T*, Hsu LW, Lai CY, Takaoka Y, Inomata M, Kitano S, Chen CL, Goto S*. Therapeutic potential of a-lipoic acid derivative, sodium zinc histidine dithiooctanamide (DHL-HisZn), in a mouse model of allergic rhinitis. International Forum of Allergy & Rhinology 2017;7(11):1095-1103.
    12. Nakano T*, Goto S, Takaoka Y, Tseng HP, Fujimura T, Kawamoto S, Ono K, Chen CL. A novel moonlight function of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for immunomodulation. Biofactors 2017 July 28. doi: 10.1002/biof.1379. [Epub ahead of print]
    13. Li LC, Hsu CN, Lin CC, Cheng YF, Hu TH, Chen DW, Lee CH, Nakano T, Chen CL. Proteinuria and baseline renal function predict mortality and renal outcomes after sirolimus therapy in liver transplantation recipients. BMC Gastroenterology 2017;17(1):58.
    14. Nakano T*, Chen IH, Goto S, Lai CY, Tseng HP, Hsu LW, Chiu KW, Lin CC, Wang CC, Cheng YF, Chen CL*. Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS 2017;21(1):55-66.
    15. Chen CC, Hsu LW, Nakano T, Huang KT, Chen KD, Lai CY, Goto S*, Chen CL*. DHL-HisZn, a novel antioxidant, enhances adipogenic differentiation and antioxidative response in adipose-derived stem cells. Biomedicine & Pharmacotherapy 2016;84:1601-9.
    16. Chiu KW, Nakano T, Chen KD, Lin CC, Hu TH, Goto S, Chen CL. Association of IL28B SNPs rs12979860 and rs8099917 on Hepatitis C Virus-RNA Status in Donors/Recipients of Living Donor Liver Transplantation. PLoS One 2016; 11(6): e0156846.
    17. Nakano T*, Kamei R, Fujimura T, Takaoka Y, Hori A, Lai CY, Chiang KC, Shimada Y, Ohmori N, Goto T, Ono K, Chen CL, Goto S, Kawamoto S*. Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice. PLoS One 2016; 11(4): e0153630.
    18. Chen KD, Huang KT, Lin CC, Weng WT, Hsu LW, Goto S, Nakano T, Lai CY, Kung CP, Chiu KW, Wang CC, Cheng YF, Ma YY, Chen CL. MicroRNA-27b Enhances the Hepatic Regenerative Properties of Adipose-Derived Mesenchymal Stem Cells. Molecular Therapy – Nucleic Acids 2016; 5(2): e285.
    19. Chiu KW, Nakano T, Chen KD, Hsu LW, Lai CY, Huang CY, Cheng YF, Goto S, Chen CL. Repeated-measures implication of hepatocellular carcinoma biomarkers in living donor liver transplantation. PLoS One 2015; 10(5): e0124943.
    20. Kusano T, Chiang KC, Inomata M, Shimada Y, Ohmori N, Goto T, Sato S, Goto S, Nakano T, Kawamoto S, Takaoka Y, Shiraishi N, Noguchi T, Kitano S. A novel anti-histone H1 monoclonal antibody, SSV monoclonal antibody, improves lung injury and survival in a mouse model of lipopolysaccharide-induced sepsis like syndrome. BioMed Research International 2015; 2015: 491649.
    21. Tsai MC, Chen KD, Wang CC, Huang KT, Wu CH, Kuo IY, Chen LY, Hu TH, Goto S, Nakano T, Dorling S, McVey JH, Chen CL, Lin CC. Factor VII promotes hepatocellular carcinoma progression through ERK-TSC signaling. Cell Death Discovery 2015;1:15051.
    22. Hsu LW, Nakano T, Chen KD, Chen CC, Lai CY, Huang KT, Yang SM, Lin CC, Wang CC, Cheng YF, Chiu KW, Kuo YR, Goto S, Chen CL. Prolonged survival by combined treatment with granulocyte colony-stimulating factor (G-CSF) and dipeptidyl peptidase IV (DPP-IV) inhibitor in a rat small-for-size liver transplantation. Hepatology Research 2015; 45(7): 804-13.
    23. Takaoka Y, Goto S, Nakano T*, Tseng HP, Yang SM, Kawamoto S, Ono K, Chen CL. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) prevents lipopolysaccharide (LPS)-induced, sepsis-related severe acute lung injury in mice. Scientific Reports 2014; 4: 5204.
    24. Chen KD, Wang CC, Tsai MC, Wu CH, Yang HJ, Chen LY, Nakano T, Goto S, Huang KT, Hu TH, Chen CL, Lin CC. Interconnections between autophagy and the coagulation cascade in hepatocellular carcinoma. Cell Death & Disease 2014; 5: e1244.
    25. Chen KD, Hsu LW, Goto S, Huang KT, Nakano T, Weng WT, Lai CY, Kuo YR, Chiu KW, Wang CC, Cheng YF, Lin CC, Ma YY, Chen CL. Regulation of heme oxygenase 1 expression by miR-27b with stem cell therapy for liver regeneration in rats. Transplantation Proceedings 2014; 46(4): 1198-200.
    26. Chen CC, Hsu LW, Nakano T, Goto S, Chen CL. Elevation of C-reactive protein level and its correlation with psychiatric comorbidities in recipients after liver transplantation. Transplantation Proceedings 2014; 46(3): 894-6.
    27. Nakano T*, Goto S, Lai CY, Hsu LW, Tseng HP, Chen KD, Chiu KW, Wang CC, Cheng YF, Chen CL*. Induction of antinuclear antibodies by de novo autoimmune hepatitis regulates alloimmune responses in rat liver transplantation. Clinical & Developmental Immunology 2013; 2013: 413928.
    28. Chiu KW, Nakano T, Chen KD, Hsu LW, Lai CY, Chiu HC, Huang CY, Cheng YF, Goto S, Chen CL. Homogeneous phenomenon of the graft when using different genotype characteristic of recipients/donors in living donor liver transplantation. World Journal of Hepatology 2013; 5(11):642-8.
    29. Hiratsuka T, Inomata M, Goto S, Oyama Y, Nakano T, Chen CL, Shiraishi N, Noguchi T, Kitano S. Phototherapy with artificial light suppresses dextran sulfate sodium-induced colitis in a mouse model. Journal of Gastroenterology and Hepatology 2014; 29(4): 749-56.
    30. Chiu KW, Nakano T, Chen KD, Lai CY, Hsu LW, Chiu HC, Huang CY, Cheng YF, Goto S, Chen CL. Pyrosequencing to identify homogenous phenomenon when using recipients/donors with different CYP3A5*3 genotypes in living donor liver transplantation. PLoS One 2013; 8(8): e71314.
    31. Chiu KW, Hu TH, Nakano T, Chen KD, Lai CY, Hsu LW, Tseng HP, Chiu HC, Cheng YF, Goto S, Chen CL. Biological interactions of CYP2C19 genotypes with CYP3A4*18, CYP3A5*3, and MDR1-3435 in living donor liver transplantation recipients. Transplantation Research 2013; 2(1): 6.
    32. Chen KD, Goto S, Hsu LW, Lin TY, Nakano T, Lai CY, Chang YC, Weng WT, Kuo YR, Wang CC, Cheng YF, Ma YY, Lin CC, Chen CL. Identification of miR-27b as a novel signature from the mRNA profiles of adipose-derived mesenchymal stem cells involved in the tolerogenic response. PLoS One 2013; 8(4): e60492.
    33. Takaoka Y, Kawamoto S, Katayama A, Nakano T, Yamanaka Y, Takahashi M, Shimada Y, Chiang KC, Ohmori N, Aki T, Goto T, Sato S, Goto S, Chen CL, Ono K. Unexpected T cell regulatory activity of anti-histone H1 autoantibody: Its mode of action in regulatory T cell-dependent and –independent manners. Biochemical and Biophysical Research Communications 2013; 431(2): 246-252.
    34. Hsu LW, Goto S, Nakano T, Chen KD, Wang CC, Lai CY, Hou CH, Chang YC, Cheng YF, Chiu KW, Chen CC, Chen SH, Chen CL. The effect of exogenous histone H1 on rat adipose-derived stem cell proliferation, migration, and osteogenic differentiation in vitro. Journal of Cellular Physiology 2012; 227(10): 3417-25.
    35. Nakano T*, Lai CY, Goto S, Hsu LW, Kawamoto S, Ono K, Chen KD, Lin CC, Chiu KW, Wang CC, Cheng YF, Chen CL*. Immunological and regenerative aspects of hepatic mast cells in liver allograft rejection and tolerance. PLoS One 2012; 7(5): e37202.
    36. Chiu KW, Nakano T, Tseng HP, Cheng YF, Jawan B, Goto S, Chen CL. CYP2C19 activity of liver tissues in Western blot analysis on living donor liver transplantation. Hepato-Gastroenterology 2012; 59(115): 805-8.
    37. Chiu KW, Nakano T, Hu TH, Tseng HP, Cheng YF, Jawan B, Eng HL, Goto S, Chen CL. Homogenous phenomenon of graft liver CYP2C19 genotypes after living donor liver transplantation. European Journal of Clinical Investigation 2012; 42(4): 352-6.
    38. Chen KD, Hsu LW, Goto S, Yeh CW, Nakano T, Lai CY, Chang YC, Hou CH, Wang CC, Cheng YF, Chiu KW, Lin CC, Chen CL. Adaptor protein Shc acts as an immune-regulator for the LPS-stimulated maturation of bone marrow-derived dendritic cells. BMC Immunology 2011; 12: 32.
    39. Nakano T, Cheng YF, Lai CY, Hsu LW, Chang YC, Deng JY, Huang YZ, Honda H, Chen KD, Wang CC, Chiu KW, Jawan B, Eng HL, Goto S, Chen CL. Impact of artificial sunlight therapy on the progress of non-alcoholic fatty liver disease in rats. Journal of Hepatology 2011; 55(2): 415-425.
    40. Chiu KW, Nakano T, Hu TH, Tseng HP, Cheng YF, Jawan B, Eng HL, Goto S, Chen CL. CYP2C19 genotypes and graft pathology after LT. Annals of Transplantation 2010; 15(4): 38-43.
    41. Huang CJ, Chen CL, Goto S, Lai CY, Kao YS, Wang CH, Eng HL, Concejero AM, Wang CC, Cheg YF, Cheng KW, Nakano T, Jawan B. Effects of hemorrhagic shock in early and late posthepatectomy rats. Transplantation Proceedings 2010; 42(3): 980-982.
    42. Chiu KW, Tai WC, Nakano T, Tseng HP, Cheng YF, Jawan B, Goto S, Chen CL. Donor graft does not affect the P450 2C19 genotype expressed in peripheral blood in recipients of living donor liver transplantation. Clinical Transplantation 2010; 24(6): 830-834.
    43. Nakano T, Goto S, Lai CY, Hsu LW, Takaoka Y, Kawamoto S, Chiang KC, Shimada Y, Ohmori N, Goto T, Sato S, Ono K, Cheng YF, Chen CL. Immunological aspects and therapeutic significance of an auto-Ab against histone H1 in a rat model of Con A-induced hepatitis. Immunology 2010; 129(4): 547-555.
    44. Kao YH, Jawan B, Goto S, Pan MC, Lin YC, Sun CK, Hsu LW, Tai MH, Cheng YF, Nakano T, Wang CS, Huang CJ, Chen CL. Serum factors potentiate hypoxia-induced hepatotoxicity in vitro through increasing transforming growth factor-beta1 activation and release. Cytokine 2009; 47(1):11-22.
    45. Chiang KC, Shimada Y, Nakano T, Lai CY, Hsu LW, Goto S, Ohmori N, Mori K, Miyagi T, Seiji Kawamoto S, Ono K, Chen CL, Goto T, Sato S. A Novel Peptide Mimotope Identified as a Potential Immunosuppressive Vaccine for Organ Transplantation. Journal of Immunology 2009; 182(7): 4282-4288.
    46. Lin YC, Goto S, Tateno C, Kao YH, Cheng YF, Jawan B, Nakano T, Hsu LW, Lai CY, Yoshizato K, Chen CL. Induction of Indoleamine 2, 3-Dioxygenase in Livers Following Hepatectomy Prolongs Survival of Allogeneic Hepatocytes after Transplantation. Transplantation Proceedings 2008; 40: 2706-2708.
    47. Kao YH, Jawan B, Goto S, Hung CT, Lin YC, Nakano T, Hsu LW, Lai CY, Tai MH, Chen CL. High-Mobility Group Box 1 Protein Activates Hepatic Stellate Cells In Vitro. Transplantation Proceedings 2008; 40: 2704-2705.
    48. Nakano T, Lai CY, Goto S, Hsu LW, Huang TL, Chen TY, Tsang LC, Chen CL, Cheng YF. Significance of portosystemic shunt on graft survival in liver transplantation: A rat model. Transplantation Proceedings 2008; 40: 2515-2516.
    49. Nakano T, Goto S, Lai CY, Hsu LW, Wong JL, Kawamoto S, Chiang KC, Ohmori N, Goto T, Sato S, Yang CH, Wang CC, Jawan B, Cheng YF, Ono K, Chen CL. Involvement of autoimmunity against nuclear histone H1 in liver transplantation tolerance. Transplant Immunology 2008; 19(2): 87-92.
    50. Hsu LW, Chen CL, Nakano T, Lai CY, Chiang KC, Lin YC, Kao YH, Chen SH, Goto T, Sung WC, Yang CH, Cheng YF, Jawan B, Chiu KW, Goto S. The role of a nuclear protein, histone H1 on signaling pathways for the maturation of dendritic cells. Clinical and Experimental Immunology 2008; 152: 576-584.
    51. Jawan B, Kao YH, Goto S, Pan MC, Lin YC, Hsu LW, Nakano T, Lai CY, Sun CK, Cheng YF, Tai MH, Eng HL, Wang CS, Huang CJ, Chen CL. Propofol pretreatment attenuates LPS-induced granulocyte-macrophage colony-stimulating factor production in cultured hepatocytes by suppressing MAPK/ERK activity and NF-B translocation. Toxicology and Applied Pharmacology 2008; 229(3): 362-373.
    52. Yamanaka Y, Kawamoto S, Katayama A, Kiso T, Aki T, Nakano T, Ohmori N, Goto T, Sato S, Chiang J, Shimada Y, Goto S, Chen CL, Ono K. Anti-histone H1 autoantibody directly acts on T cells to exert its immunosuppressive activity. Animal Cell Technology: Basic & Applied Aspects 2008; 15: 153-158.
    53. Katayama A, Kawamoto S, Yamanaka Y, Kiso T, Aki T, Nakano T, Ohmori N, Goto T, Sato S, Chiang J, Shimada Y, Goto S, Chen CL, Ono K. Anti-histone H1 autoantibody: An inducible immunosuppressive factor in liver transplantation. Animal Cell Technology: Basic & Applied Aspects 2008; 15: 145-151.
    54. Lin YC, Chen CL, Nakano T, Goto S, Kao YH, Hsu LW, Lai CY, Jawan B, Cheng YF, Tateno C, Yoshizato K. The immunological role of indoleamine 2, 3-dioxygenase in rat liver allograft rejection and tolerance. Journal of Gastroenterology and Hepatology 2008; 23(7 Pt 2): e243-50.
    55. Shimada Y, Goto T, Kawamoto S, Kiso T, Katayama A, Yamanaka Y, Aki T, Chiang KC, Nakano T, Goto S, Chen CL, Ohmori N, Ono K, Sato S. Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Biomedical Chromatography 2008; 22: 13-19.
    56. Kao YH, Goto S, Jawan B, Nakano T, Hsu LW, Lin YC, Pan MC, Lai CY, Sun CK, Cheng YF, Tai MH, Huang HT, Chen CL. Heat preconditioning ameliorates hepatocyte viability after cold preservation and rewarming, and modulates its immunoactivity. Transplant Immunology 2008; 18(3): 220-231.
    57. Hsu LW, Yang CH, Goto S, Nakano T, Lai CY, Lin YC, Kao YH, Chen SH, Cheng YF, Jawan B, Chiu KW, Chen CL. The effects of suplatast tosilate (IPD-1151T) on innate immunity and antigen presenting cells. Transplant Immunology 2007; 18(2): 108-114.
    58. Nakano T, Goto S, Lai CY, Hsu LW, Kao YH, Lin YC, Kawamoto S, Chiang KC, Ohmori N, Goto T, Sato S, Jawan B, Cheng YF, Ono K, Chen CL. Experimental and clinical significance of anti-nuclear antibodies in liver transplantation. Transplantation 2007; 83(8): 1122-1125.
    59. Nakano T, Goto S, Lai CY, Hsu LW, Ono K, Kawamoto S, Lin YC, Kao YH, Chiang KC, Ohmori N, Goto T, Sato S, Tu CH, Jawan B, Cheng YF, Chen CL. Impact of vaccine therapy using nuclear histone H1 on allograft survival in experimental organ transplantation. Transplant Immunology 2007; 17(3): 147-152.
    60. Hsu LW, Goto S, Nakano T, Lai CY, Lin YC, Kao YH, Chen SH, Cheng YF, Jawan B, Chiu KW, Chen CL. Immunosuppressive activity of serum taken from a liver transplant recipient after withdrawal of immunosuppressants. Transplant Immunology 2007; 17(2): 137-146.
    61. Nakano T, Chen CL, Goto S, Lai CY, Hsu LW, Kawamoto S, Sasaki T, Lin YC, Kao YH, Ohmori N, Goto T, Sato S, Jawan B, Ono K, Cheng YF. The immunological role of lipid transfer/metabolic proteins in liver transplantation tolerance. Transplant Immunology 2007; 17(2): 130-136.
    62. Nakano T, Lai CY, Goto S, Hsu LW, Lin YC, Kao YH, Kawamoto S, Chiang KC, Ohmori N, Goto T, Sato S, Ono K, Jawan B, Cheng YF, Chen CL. The role of anti-nuclear antibodies in experimental and clinical liver transplantation. Transplantation Proceedings 2006; 38(10): 3605-3606.
    63. Nakano T, Ono K, Goto S, Lai CY, Hsu LW, Kawamoto S, Lin YC, Kao YH, Chiang KC, Ohmori N, Goto T, Sato S, Jawan B, Cheng YF, Chen CL. Histone H1 vaccine therapy for overcoming the acute rejection in experimental organ transplantation. Transplantation Proceedings 2006; 38(10): 3247-3248.
    64. Hsu LW, Goto S, Nakano T, Lai CY, Kao YH, Lin YC, Kawamoto S, Ono K, Lord R, Goto T, Ohmori N, Sato S, Chiang KC, Chen SH, Jawan B, Cheng YF, Chiu KW, Chen CL. The effects of anti-histone H1 antibody on immune cells responsible for rejection reaction. Molecular Immunology 2005; 42(10): 1155-1164.
    65. Nakano T, Kawamoto S, Lai CY, Hsu LW, Lin YC, Sasaki T, Aki T, Shigeta S, Goto T, Ohmori N, Sato S, Goto S, Ono K, Chen CL. Characterization of immunosuppressive factors expressed in serum by rat tolerogenic liver transplantation. Transplantation Proceedings 2005; 37(1): 80-81.
    66. Onishi N, Kawamoto S, Nishimura M, Nakano T, Aki T, Shigeta S, Shimizu H, Hashimoto K, Ono K. A new immunomodulatory function of low-viscous konjac glucomannan with a small particle size: its oral intake suppresses spontaneously occurring dermatitis in NC/Nga mice. International Archives of Allergy and Immunology 2005; 136 (3): 258-265.
    67. Onishi N, Kawamoto S, Nishimura M, Nakano T, Aki T, Shigeta S, Shimizu H, Hashimoto K, Ono K. The ability of konjac-glucomannan to suppress spontaneously occurring dermatitis in NC/Nga mice depends upon the particle size. BioFactors 2004; 21(1-4): 163-166.
    68. Nakano T, Kawamoto S, Lai CY, Sasaki T, Aki T, Shigeta S, Goto T, Sato S, Goto S, Chen CL, Ono K. Liver transplantation-induced antihistone H1 autoantibodies suppress mixed lymphocyte reaction. Transplantation 2004; 77(10): 1595-1603.
    69. Nakano T, Kawamoto S, Sasaki T, Maekawa K, Aki T, Shigeta S, Suzuki O, Goto S, Ono K. Analysis of immunosuppressive factors expressed in liver-transplanted rats. Animal Cell Technology: Basic & Applied Aspects 2002; 12: 257-261.

     

    • Review
    1. Nakano T*, Chiang KC, Chen CC, Chen PJ, Lai CY, Hsu LW, Ohmori N, Goto T, Chen CL, Goto S*. Sunlight Exposure and Phototherapy: Perspectives for Healthy Aging in an Era of COVID-19. Int J Environ Res Public Health. 2021;18(20):10950.
    2. Goto S, Nakano T, Chen CL, Chiu KW, Hsu LW, I S, Chen IH, Huang KT, Chen DW, Goto T, Omori N, Sato S, Kai H, Tsuruta W, Kai M, Bahau SP, Takaoka Y, Tane E, Yuyama K, Wano C, Inoue E, Lord R, Iida K. Application of artificial sunlight for the elderly as a possible environmental nursing practice. POJ Nursing Practice & Research 2018;2(1):1-5.
    3. Goto S*, Nakano T*, Hsu LW, Chiang KC, Shimada Y, Goto T, Ohmori N, Sato S, Takaoka Y, Magari Y, Kawamoto S, Chen CL*. The Significance of a Nuclear Protein as a Moonlight Protein: Diagnostic and Therapeutic Potentials in Liver Transplantation. International Journal of Surgery & Surgical Procedures 2017;2:117.
    4. Chiu KW, Nakano T, Chen KD, Hsu LW, Lai CY, Huang CY, Cheng YF, Goto S, Chen CL. Cytochrome P450 in living donor liver transplantation. Journal of Biomedical Science 2015; 22: 32.
    5. Nakano T*, Chen CL, Goto S*. Nuclear antigens and auto/alloantibody responses: Friend or foe in transplant immunology. Clinical & Developmental Immunology 2013; 2013: 267156.
    6. Goto S, Nakano T, Hsu LW, Chiang KC, Lai CY, Kawamoto S, Ono K, Chen KD, Ohmori N, Goto T, Sato S, Kuo YR, Wang CC, Jawan B, Cheng YF, Chen CL. Autoimmunity and liver transplantation immunology. Current Trends in Immunology 2011; 12: 1-11.

     

    • Invited Lecture
    1. Nakano T. The war on cancer: Mechanisms of tumor escape from immune surveillance. 6thHiHA International Symposium, Higashi-Hiroshima, Japan, 8 March 2019.
    2. Nakano T. Histones and GAPDH: Moonlight Functions for Rejection and Tolerance. Transplantation Science Symposium Asian Regional Meeting 2018, Taipei, Taiwan, 25 Nov. 2018.
    3. Nakano T. Sunlight and vitamin D for healthy aging.5thHiHA International Symposium, Higashi-Hiroshima, Japan, 12 March 2018.
    4. Nakano T. Histones and GAPDH: moonlight functions for immunoregulation and tolerance. Hiroshima Research Center for Healthy Aging (HiHA) 4th International Symposium, Higashi-Hiroshima, Japan, 10 March 2017.
    5. Nakano T. Circulating exosomes: key factor for hepatocellular carcinoma (HCC) development and recurrence. Hiroshima Research Center for Healthy Aging (HiHA) 3rd International Symposium, Higashi-Hiroshima, Japan, 11 March 2016.
    6. Nakano T. The role of nuclear antigens and its application for therapeutics in type I hypersensitivity. Hiroshima Research Center for Healthy Aging (HiHA) 2nd International Symposium, Higashi-Hiroshima, Japan, 13 March 2015.
    7. 中野敏明. トランスレーショナルアプローチによる肝移植拒絶診断バイオマーカーの探索. 広島大学健康長寿研究拠点（HiHA）第三回ワークショップ, 東広島, 2014年12月26日
    8. Nakano T. Translational research in liver and transplant immunology. Hiroshima Research Center for Healthy Aging (HiHA) International Symposium, Higashi-Hiroshima, Japan, 7 March 2014.
    9. 中野敏明, 後藤茂, Hsu LW, Lai CY, Chen CL. aリポ酸誘導体（DHLHZn）のアレルギーおよび移植免疫分野への応用に向けて. 第二回 癌・炎症とaリポ酸研究会（CIA研究会）, 別府, 2011年11月11-12日

     

    • Congress Presentation (International)
    1. Nakano T, Chen IH, Tseng HP, Chen BL, Lin CC, Cheng YF, Goto S, Chen CL. Strategic release of exosomal microRNAs in hepatocellular carcinoma for generation of an immunosuppressive tumor microenvironment. ILTS 25th Annual International Congress, Toronto, Canada, 14-18 May 2019. (Oral)
    2. Nakano T, Goto S, Chen IH, Tseng HP, Lai CY, Hsu LW, Lin CC, Wang CC, Cheng YF, Chen CL. Immune surveillance vs. immune escape: Strategic release of exosomal microRNAs in hepatocellular carcinoma. The 2018 Joint International Congress of ILTS, ELITA & LICAGE, Lisbon, Portugal, 23-26 May 2018. (Oral)
    3. Nakano T, Goto S, Tseng HP, Fujimura T, Kawamoto S, Chen CL. A novel moonlighting function of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for immunomodulation. Consortium of Biological Sciences (ConBio2017), Kobe, Japan, 6-9 December 2017. (Poster)
    4. Nakano T, Goto S, Tseng HP, Chen IH, Chen CL. A novel moonlighting function of extracellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for cancer immunoediting. ILCA2017 International Liver Cancer Association 11th Annual Conference, Seoul, South Korea, 15-17 Sep. 2017. (e-Poster)
    5. Nakano T, Goto S, Lai CY, Hsu LW, Chen IH, Lin CC, Chiu KW, Wang CC, Cheng YF, Chen CL. Omics and translational approaches for appropriate biomarker discovery in liver transplantation. The 2017 Joint International Congress of ILTS, ELITA & LICAGE, Prague, Czech Republic, 24-27 May 2017. (Poster)
    6. Nakano T, Goto S, Goto T, Kawamoto S, Chen CL. Histone H1: a novel alarmin-like mediator and molecular target for immune regulation. The 13th International Workshop on Autoantibody and Autoimmunity (IWAA 2016), Kyoto, Japan, 11-13 Oct. 2016. (Poster)
    7. Fujimura T, Nakano T, Goto S, Goto T, Ono K, Chen CL, Kawamoto S. Extracellular linker histone 1 deteriorates IgE-mediated allergic hypersensitivity. The 13th International Workshop on Autoantibody and Autoimmunity (IWAA 2016), Kyoto, Japan, 11-13 Oct. 2016 (Poster)
    8. Hsu LW, Chen CC, Goto S, Huang KT, Nakano T, Chen DW, Lin CC, Chen CL. DHL-HisZn Attenuates Fatty Acid Uptake and Lipid Accumulation Through Activation of AMPK and Down-regulation of CD36 in HepG2 Cells. The 2016 Joint International Congress of International Liver Transplantation Society (ILTS), Seoul, Korea, 4-7 May 2016.(Poster)
    9. Chen KD, Huang KT, Lin CC, Weng WT, Hsu LW, Goto S, Nakano T, Lai CY, Kung CP, Chiu KW, Wang CC, Cheng YF, Ma YY, Chen CL. Micro-RNA 27b Enhances the Hepatic Regenerative Properties of Adipose-Derived Mesenchymal Stem Cells. The 2016 Joint International Congress of International Liver Transplantation Society (ILTS), Seoul, Korea, 4-7 May 2016. (Poster)
    10. Chiu KW, Nakano T, Lin CC, Hu TH, Goto S, Chen CL. The Association between IL28B SNPs rs8099917 and rs12979860 with Sporadic Genotyping Change after Living Donor Liver Transplant for Patients with Chronic C Hepatitis. The 2016 Joint International Congress of International Liver Transplantation Society (ILTS), Seoul, Korea, 4-7 May 2016. (Poster)
    11. Chen IH, Nakano T, Goto S, Lin CC, Chiu KW, Wang CC, Cheng YF, Chen CL. Circulating Exosomes: Key Factors for HCC Recurrence after Liver Transplantation. The 2016 Joint International Congress of International Liver Transplantation Society (ILTS), Seoul, Korea, 4-7 May 2016. (Poster)
    12. Goto S, Nakano T, Hsu LW, Lai CY, Chen IH, Cheng YF, Chiu KW, Chen CL. Artificial Sunlight and Vitamin D Ameliorate Non-Alcoholic Steatohepatitis (NASH). The 2016 Joint International Congress of International Liver Transplantation Society (ILTS), Seoul, Korea, 4-7 May 2016. (Poster)
    13. Nakano T, Chen IH, Lai CY, Tseng HP, Yang SM, Hsu LW, Goto S, Chen CL. Hepatic miR-301a acts as a key immune regulator for T-helper 17-mediated lethal acute rejection in experimental liver transplantation. Transplantation Science Symposium (TSS) Asian Regional Meeting 2016, Tokyo, Japan, 8-9 April 2016. (Poster)
    14. Nakano T, Goto S, Chen CL. Histone H1: a novel alarmin-like endogenous mediator and molecular target for immune regulation. 2015 NHRI/IBMS Joint International Conference on Inflammation & Disease, Miaoli, Taiwan, 21-23 October 2015. (Poster)
    15. Nakano T, Chen IH, Goto S, Lin CC, Chiu KW, Wang CC, Cheng YF, Chen CL. Circulating exosomes: key factors for HCC recurrence after liver transplantation. 14th Congress of The Asian Society of Transplantation (CAST 2015), Singapore, 23-26 August 2015. (Poster)
    16. Nakano T, Chen IH, Goto S, Lai CY, Tseng HP, Chiu KW, Lin CC, Chen CL. Hepatic miR-301a acts as a diagnostic biomarker for acute rejection in experimental liver transplantation. ILTS 21st Annual International Congress. Chicago, IL, USA, 8-11 July 2015. (Poster)
    17. Lai CY, Nakano T, Goto S, Yang SM, Tseng HP, Lin CC, Cheng YF, Chen CL. Exhaled breath nitric oxide test as a noninvasive diagnosis of rejection in experimental liver transplantation. ILTS 21st Annual International Congress. Chicago, IL, USA, 8-11 July 2015. (Poster)
    18. Goto S, Hsu LW, Nakano T, Lai CY, Takaoka Y, Chen KD, Chiu KW, Lin CC, Wang CC, Cheng YF, Chen CL. Significance of post-liver transplantation induced anti-histone H1 antibody for prevention of chronic rejection and liver fibrosis. The World Transplant Congress (WTC) 2014, San Francisco, CA, USA, 26-31 July 2014. (Poster)
    19. Nakano T, Goto S, Lai CY, Hsu LW, Takaoka Y, Chiu KW, Lin CC, Wang CC, Cheng YF, Chen CL. Translational approach to predictive biomarker discovery in liver transplant rejection. The World Transplant Congress (WTC) 2014, San Francisco, CA, USA, 26-31 July 2014. (Poster)
    20. Takaoka Y (Mentee), Nakano T (Mentor), Goto S, Lai CY, Hsu LW, Tseng HP, Chen KD, Chiu KW, Wang CC, Cheng YF Chen CL. Autoimmunity vs. alloimmunity: its involvement in liver transplant tolerogenicity. 3rd ESOT Basic Science Meeting/13th TTS Basic Science Symposium, Paris, France, 7-9 November 2013. (Poster: TTS-ILTS International Basic Science Mentee-Mentor Award)
    21. Chen KD, Goto S, Lin TY, Weng WT, Nakano T, Lai CY, Lin CC, Wang CC, Chen CL. Regulation of heme oxygenase-1 expression by miR-27 on stem cell therapy for liver regeneration in rats. The 13th Congress of the Asian Society of Transplantation, Kyoto, Japan, 2-6 September 2013. (Poster)
    22. Hsu LW, Goto S, Nakano T, Lai CY, Chen CL. The effect o nuclear histone H1 on rat liver fibrosis and hepatic stellate cells activation. The 13th Congress of the Asian Society of Transplantation, Kyoto, Japan, 2-6 September 2013. (Poster)
    23. Chiu KW, Nakano T, Chen KD, Goto S, Chen CL. Pyrosequencing further confirmation the homogenous phenomenon of CYP3A4*3 when the different genotype of SNP between the recipients and donors in living donor liver transplantation. The 13th Congress of the Asian Society of Transplantation, Kyoto, Japan, 2-6 September 2013. (Poster)
    24. Goto S, Hsu LW, Nakano T, Takaoka Y, Lai CY, Kuo YR. Chen KD, Chiu KW, Lin CC, Wang CC, Cheng YF, Chen CL. The Role of Dipeptidylpeptidase 4 (DPP4) in Liver Transplantation. The 13th Congress of the Asian Society of Transplantation, Kyoto, Japan, 2-6 September 2013. (Poster)
    25. Nakano T, Goto S, Lai CY, Hsu LW, Takaoka Y, Chen KD, , Chiu KW, , Lin CC, , Wang CC, , Cheng YF , Chen CL. Proteomic and Translational Approaches for Biomarker Discovery in Liver Transplant Rejection. The 13th Congress of the Asian Society of Transplantation, Kyoto, Japan, 2-6 September 2013. (Oral)
    26. Nakano T, Goto S, Lai CY, Chang YC, Hsu LW, Tseng HP, Kang HJ, Wang CC, Cheng YF, Chen CL. Induction of anti-nuclear antibodies regulates alloimmune responses in experimental liver transplantation. ILTS 19th Annual International Congress, Sydney, Australia, 12-15 June 2013. (Poster)
    27. Chen KD, Goto S, Nakano T, Lin TY, Weng WT, Lai CY, Wang CC, Cheng YF, Kuo YR, Chen CL. Regulation of heme oxygenase-1 expression by miR-27b on stem cell therapy for liver regeneration in rats. ILTS 19th Annual International Congress, Sydney, Australia, 12-15 June 2013. (Poster)
    28. Chiu KW, Nakano T, Goto S, Hu TH, Chen CL. Graft liver CYP2C19 genotypes and post-operative abnormal liver function after living donor liver transplantation. ILTS 18th Annual International Congress, San Francisco, CA, USA, 16-19 May 2012. (Poster)
    29. Goto S, Hsu LW, Chang YC, Nakano T, Chen KD, Lai CY, Wang CC, Cheng YF, Chiu KW, Lin CC, Chen CL. Treatment with DPP-IV inhibitor and G-CSF may enhance mobilization of host stem cells and contribute to overcome small-for-size graft syndrome. ILTS 18th Annual International Congress, San Francisco, CA, USA, 16-19 May 2012. (Poster)
    30. Lin CC, Yang HJ, Chen LY, Wang CC, Chen KD, Nakano T, Goto S, Chen CL.Coagulation factor VII expression predicts the vascular invasion in human hepatocellular carcinoma. ILTS 18th Annual International Congress, San Francisco, CA, USA, 16-19 May 2012. (Poster)
    31. Nakano T, Goto S, Lai CY, Hsu LW, Chen KD, Lin CC, Wang CC, Cheng YF, Chen CL. Immunological and regenerative aspects of hepatic mast cells in liver allograft rejection and tolerance. ILTS 18th Annual International Congress, San Francisco, CA, USA, 16-19 May 2012. (Poster)
    32. Lai CY, Nakano T, Goto S, Chang YC, Hsu LW, Chen KD, Lin CC, Wang CC, Cheng YF, Chen CL. Induction of autoimmune hepatitis paradoxically reduce the rejection after liver transplantation in rats. ILTS 18th Annual International Congress, San Francisco, CA, USA, 16-19 May 2012. (Poster)
    33. Chen KD, Goto S, Hsu LW, Nakano T, Lai CY, Chang YC, Lin TY, Kuo YR, Wang CC, Cheng YF, Chen CL. The role of miR-27 in affecting immunosuppressive activity of adipose-derived mesenchymal stem cells by regulating the expression of heme oxigenase-1. ILTS 18th Annual International Congress, San Francisco, CA, USA, 16-19 May 2012. (Poster)
    34. Goto S, Nakano T, Lai CY, Chang YC, Hsu LW, Chen KD, Lin CC, Wang CC, Chiu KW, Cheng YF, Chen CL. Induction of autoimmune hepatitis paradoxically reduce the rejection after liver transplantation in rats. The 5th Asian Congress on Autoimmunity, Singapore, 17-19 November 2011. (Oral)
    35. Nakano T, Goto S, Lai CY, Hsu LW, Chen KD, Lin CC, Cheng YF, Chen CL. Dynamics of mast cells, regulatory T cells and hepatic stem/progenitor cells in liver allograft rejection and tolerance. 12th Congress of the Asian Society of Transplantation, Seoul, Korea, 25-28 September 2011. (Oral)
    36. Chiu KW, Nakano T, Hu TH, Tseng HP, Cheng YF, Jawan B, Eng HL, Goto S, Chen CL. The influence of CYP2C19 genotype in liver tissue between the donor and recipient after living donor liver transplantation. The 2011 Joint International Congress of ILTS, ELITA, & LICAGE, Valencia, Spain, 22-25 June 2011.
    37. Nakano T, Goto S, Lai CY, Hsu LW, Chang YC, Chen KD, Cheng YF, Chen CL. Impact of artificial sunlight therapy on the progress of non-alcoholic fatty liver disease in rats. The 2011 Joint International Congress of ILTS, ELITA, & LICAGE, Valencia, Spain, 22-25 June 2011. (Poster)
    38. Chen KD, Yeh CW, Goto S, Nakano T, Kuo YR, Hsu LW, Chang YC, Wang CC, Lin CC, Chiu KW, Cheng YF, Chen CL. Role of miR-27b in LPS responsiveness of adipocyte-derived mesenchymal stem cells from experimental liver transplantation models. The 2011 Joint International Congress of ILTS, ELITA, & LICAGE, Valencia, Spain, 22-25 June 2011.
    39. Hsu LW, Chen CL, Hou CH, Nakano T, Goto S. The role of innate response on allograft rejection and tolerance. ILTS 16th Annual International Congress, Hong Kong, China, 16-19 June 2010. (Poster)
    40. Chiu KW, Nakano T, Cheng YF, Goto S, Chen CL. CYP2C19 genotypes and graft pathology in recipients after liver transplantation. ILTS 16th Annual International Congress, Hong Kong, China, 16-19 June 2010.
    41. Nakano T, Lai CY, Goto S, Hsu LW, Deng JY, Huang YZ, Chang YC, Chen KD, Cheng YF, Chen CL. Immunological aspects of hepatic mast cells in liver allograft tolerance. ILTS 16th Annual International Congress, Hong Kong, China, 16-19 June 2010. (Poster)
    42. Nakano T, Huang YZ, Hsu LW, Lai CY, Goto S, Hsiao CC, Yang KD, Chen CL. Biomarker discovery in experimental and clinical liver allograft rejection and tolerance. 2010 Disease Biomarker and TPS International Conference, Chang Gung University, Tao-Yuan, Taiwan, 23-24 April 2010. (Poster)
    43. Goto S, Hsu LW, Nakano T, Lai CY, Cheng YF, Chen CL. Proteomics in experimental and clinical liver allograft tolerance. 1st International Conference on Transplantomics and Biomarkers in Organ Transplantation, San Francisco, CA, USA, 24-26 February 2010. (Poster)
    44. Nakano T, Lai CY, Goto S, Hsu LW, Chiang KC, Ono K, Kawarasaki H, Chen CL. Anti-nuclear autoantibodies as biomarkers in liver transplantation tolerance. 1st International Conference on Transplantomics and Biomarkers in Organ Transplantation, San Francisco, CA, USA, 24-26 February 2010.
    45. Goto S, Nakano T, Kawamoto S, Ono K, Chiang KC, Lai CY, Hsu LW, Ohmori N, Goto T, Sato S, Wang CC, Jawan B, Cheng YF, Chen CL. Immunosuppressive autoimmune antibody against a nuclear protein (histone H1) in liver allograft tolerance. The 4th Asian Congress on Autoimmunity, Singapore, 11-13 September 2009. (Oral)
    46. Nakano T, Goto S, Lai CY, Hsu LW, Yang CH, Wang CC, Jawan B, Cheng YF, Chen CL. Therapeutic significance of autoantibody against histone H1 in a rat model of T-cell mediated autoimmune hepatitis. ILTS 15th Annual International Congress, New York, NY, USA, 8-11 July 2009. (Poster)
    47. Hsu LW, Chen CL, Nakano T, Lai CY, Goto S, Wong JL, Chung YC. The role of a nuclear protein, histone H1 on signaling pathways for the maturation of dendritic cells. The 4th Congress of the Federation of Immunology Societies of Asia-Oceania (FIMSA 2008), Taipei, Taiwan, 17-20 October 2008. (Poster)
    48. Nakano T, Goto S, Hsu LW, Lai CY, Wong JL, Chen CL. Autoimmunity and tolerance induction in experimental and clinical liver transplantation. The 4th Congress of the Federation of Immunology Societies of Asia-Oceania (FIMSA 2008), Taipei, Taiwan, 17-20 October 2008. (Poster)
    49. Hsu LW, Goto S, Nakano T, Lai CY, Chen SH, Chiang KC, Goto T, Yang CH, Wang CC, Chen CL. A novel role of nuclear protein, histone H1 on transplantation immunology. XXII International Congress of The Transplantation Society, Sydney, Australia, 10-14 August 2008. (Poster)
    50. Chen YF. Nakano T, Lai CY, Hsu LW, Wong JL, Chung YC, Huang TL, Eng HL, Goto S, Chen CL. Characterization of lipid transfer/metabolic proteins in a rat non-alcoholic steatohepatitis model. XXII International Congress of The Transplantation Society, Sydney, Australia, 10-14 August 2008. (Poster)
    51. Goto S, Nakano T, Hsu LW, Lai CY, Kawamoto S, Chiang KC, Goto T, Ono K, Lynch SV, Chen CL. Liver suppressor factors specific to autoimmunity in liver transplantation. XXII International Congress of The Transplantation Society, Sydney, Australia, 10-14 August 2008. (Poster)
    52. Lai CY, Nakano T, Goto S, Hsu LW, Wong JL, Kawamoto S, Goto T, Ono K, Cheng YF, Chen CL. Protection and recovery from autoimmune hepatitis by anti-histone H1 autoantibody. XXII International Congress of The Transplantation Society, Sydney, Australia, 10-14 August 2008. (Poster)
    53. Nakano T, Goto S, Hsu LW, Lai CY, Wong JL, Kawamoto S, Goto T, Ono K, Cheng YF, Chen CL. The immunological aspects of anti-histone H1 autoantibody on lymphocyte biology in liver transplantation tolerance. XXII International Congress of The Transplantation Society, Sydney, Australia, 10-14 August 2008. (Poster)
    54. Cheng YF, Nakano T, Goto S, Lai CY, Hsu LW, Wong JL, Eng HL, Che CL. Establishment of noninvasive approach for diagnosis of non-alcoholic steatohepatitis in rats. The 2008 Joint International Congress of ILTS, ELITA and LICAGE, Paris, France, 9-12 July 2008. (Poster)
    55. Kao YH, Jawan B, Goto S, Hung CT, Lin YC, Nakano T, Tai MH, Chao D, Chen CL. High-mobility group box 1 protein activates hepatic stellate cells in vitro. 10th Congress of the Asian Society of Transplantation, Pattaya, Thailand, 1-4 December 2007. (Poster)
    56. Lin YC, Goto S, Nakano T, Jawan B, Cheng YF, Tateno C, Yoshizato K, Chen CL. Induction of Indoleamine 2,3-dioxygenase (IDO) in livers following hepatectomy prolongs survival of allogeneic hepatocytes after transplantation. 10th Congress of the Asian Society of Transplantation, Pattaya, Thailand, 1-4 December 2007. (Poster)
    57. Cheng YF, Nakano T, Lai CY, Goto S, Hsu LW, Huang TL, Chen TY, L-C. Tsang LC, Chen CL. Significance of portosystemic shunt on graft survival in liver transplantation. 10th Congress of the Asian Society of Transplantation, Pattaya, Thailand, 1-4 December 2007. (Poster)
    58. Chen YF, Lai CY, Nakano T, Goto S, Hsu LW, Huang TL, Chen TY, Tsang LC, Chen CL. The effects of portosystemic shunt on graft survival and hemodynamics in rat liver transplantation. The 13th Congress of the European Society for Organ Transplantation, Prague, Czech Republic, September - 3 October 2007. (Poster)
    59. Hsu LW, Goto S, Nakano T, Lai CY, Chiang KC, Lin YC, Kao YH, Goto T, Chen SH, Yang CH, Jawan B, Cheng YF, Chiu KW, Ono K, and Chen CL. Significance of histone H1 for the maturation of dendritic cells and subsequent T-cell activation. The 13th Congress of the European Society for Organ Transplantation, Prague, Czech Republic, September - 3 October 2007. (Poster)
    60. Nakano T, Goto S, Lai CY, Hsu LW, Lin YC, Kao YH, Kawamoto S, Ono K, Chiang KC, Ohmori N, Goto T, Sato S, Jawan B, Cheng YF, and Chen CL. Autoimmunity and tolerance induction in rat liver transplantation models. The 13th Congress of the European Society for Organ Transplantation, Prague, Czech Republic, 30 September - 3 October 2007. (Poster)
    61. Chiang KC, Goto T, Ohmori N, Shimada Y, Goto S, Kawamoto S, Ono K, Yamanaka Y, Nakano T, Lai CY, Hsu LW, Chen CL, and Sato S. Identification and characterization of peptides binding to a novel histone H1 monoclonal antibody by phage display. 34th Annual meeting & Exposition of the Controlled Release Society, Long Beach, CA, USA, 7-11 July 2007. (Poster)
    62. Cheng YF, Nakano T, Goto S, Lai CY, Hsu LW, Kawamoto S, Lin YC, Kao YH, Chiang KC, Ohmori N, Goto T, Sato S, Jawan B, Ono K, and Chen CL. The immunological role of lipid transfer/metabolic proteins in liver transplantation tolerance. 13th International Liver Transplant Society Annual Congress, Rio de Janeiro, Brazil, 20-23 June 2007. (Poster)
    63. Kawamoto S, Katayama A, Yamanaka Y, Kiso T, Aki T, Nakano T, Chiang KC, Shimada Y, Ohmori N, Goto T, Sato S, Goto S, Chen CL, and Ono K. Mode of action of anti-histone H1 autoantibody in the induction of liver transplantation tolerance. World Transplant Congress 2006, Boston, MA, USA, 22-27 July 2006. (Poster)
    64. Nakano T, Ono K, Goto S, Lai CY, Hsu LW, Kawamoto S, Lin YC, Kao YH, Chiang KC, Ohmori N, Goto T, Sato S, Ono K, Jawan B, Cheng YF, and Chen CL. Histone H1 vaccine therapy for overcoming the acute rejection in experimental organ transplantation. World Transplant Congress 2006, Boston, MA, USA, 22-27 July 2006. (Poster)
    65. Lai CY, Nakano T, Goto S, Hsu LW, Lin YC, Kao YH, Kawamoto S, Chiang KC, Ohmori N, Goto T, Sato S, Ono K, Jawan B, Cheng YF, and Chen CL. The role of anti-nuclear antibodies in experimental and clinical liver transplantation. World Transplant Congress 2006, Boston, MA, USA, 22-27 July 2006. (Poster)
    66. Nakano T, Kawamoto S, Lai CY, Sasaki T, Aki T, Shigeta S, Goto T, Goto S, Ono K, and Chen CL. Characterization of immunosuppressive factors expressed in serum by rat tolerogenic liver transplantation. XX International Congress of The Transplantation Society, 5-10 Sep. 2004. (Poster)
    67. Nakano T, Kawamoto S, Sasaki T, Maekawa K, Aki T, Shigeta S, Goto S, and Ono K. Immunosuppressive autoreactive antibodies induced by liver transplantation. 11th International Congress of Immunology, Stockholm, Sweden, 22-27 July 2001. (Poster)
    68. Nakano T, Kawamoto S, Sasaki T, Maekawa K, Aki T, Shigeta S, Suzuki O, Goto S, and Ono K. Analysis of immunosuppressive factors expressed in serum of liver transplanted rats. The Thirteenth Annual Meeting of the Japanese Association for Animal Cell Technology, Fukuoka, Japan, 17-21 November 2000. (Poster)

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